Optimization of Crystalline Xylose Production from Coffee Cherry Processing Waste





Functional Food, Wood Sugar, Process Engineering, Sucrase, Xylanase


Purpose: This study aims to optimize the production of crystalline xylose from coffee cherry processing waste (CCPW) by investigating the influence of substrate concentration (SC), xylanase concentration (XC), and commercial xylose crystal concentration (CXC) on the process.


Theoretical Reference: The research utilizes Response Surface Methodology (RSM) to design experiments and analyze the effects of SC, XC, and CXC on crystalline xylose production.


Method: Experimental laboratory procedures were conducted with varying levels of SC (10-20%), XC (1-5%), and CXC (2-7%). Xylose production was carried out in fed-batch mode, with substrate feeding every 12 hours. Solvent volume was maintained at 250ml, with 60% added initially and the remaining 40% distributed incrementally during production.


Results: The study found significant effects of XC and SC on xylose production, while CXC showed no significant influence. The optimized conditions were determined with SC at 10%, XC at 5%, and CXC at 7%, resulting in a xylose content of 0.804 g/L and a desirability value of 0.632. The model's validity was confirmed with a validation accuracy of 98.99%.


Conclusion: The research demonstrates the effectiveness of RSM in optimizing crystalline xylose production from CCPW, highlighting the importance of XC and SC levels in the process.


Implications of Research: The optimized conditions identified in this study offer practical insights for enhancing xylose production efficiency from CCPW, potentially contributing to the development of functional food ingredients and process engineering applications.


Originality/Value: This study contributes to the field by providing a systematic approach to maximizing xylose production from coffee cherry processing waste, offering valuable knowledge for both academia and industry.


Download data is not yet available.


F. J. Cerino-Córdova, N. E. Dávila-Guzmán, A. M. García León, J. J. Salazar-Rabago, and E. Soto-Regalado, “Revalorization of Coffee Waste,” in Coffee - Production and Research, IntechOpen, 2020. doi: 10.5772/intechopen.92303.

Y.-G. Lee, E.-J. Cho, S. Maskey, D.-T. Nguyen, and H.-J. Bae, “Value-Added Products from Coffee Waste: A Review,” Molecules, vol. 28, no. 8, p. 3562, Apr. 2023, doi: 10.3390/molecules28083562.

D. M. Rahmah, E. Mardawati, R. Kastaman, T. Pujianto, and R. Pramulya, “Coffee Pulp Biomass Utilization on Coffee Production and Its Impact on Energy Saving, CO2 Emission Reduction, and Economic Value Added to Promote Green Lean Practice in Agriculture Production,” Agronomy, vol. 13, no. 3, Mar. 2023, doi: 10.3390/agronomy13030904.

G. Gómez Millán, R. P. Bangalore Ashok, P. Oinas, J. Llorca, and H. Sixta, “Furfural production from xylose and birch hydrolysate liquor in a biphasic system and techno-economic analysis,” Biomass Convers Biorefin, vol. 11, no. 5, pp. 2095–2106, Oct. 2021, doi: 10.1007/s13399-020-00702-4.

Badan Pembangunan Nasional Republik Indonesia, “Lampiran Peraturan Presiden Republik Indonesia Nomor 18 Tahun 2020 Tentang Rencana Pembangunan Jangka Menengah Nasional 2020-2024 Narasi Republik Indonesia,” Jakarta, 2020.

T. Wang and X. Lu, “Chapter 8 - Overcome saccharification barrier: Advances in hydrolysis technology,” in Advances in 2nd Generation of Bioethanol Production, 1st ed., vol. 2, X. Lu, Ed., Sawston: Woodhead Publishing, 2021, pp. 137–159.

M. S. Pino et al., “Bioreactor design for enzymatic hydrolysis of biomass under the biorefinery concept,” Chemical Engineering Journal, vol. 347. Elsevier B.V., pp. 119–136, Sep. 01, 2018. doi: 10.1016/j.cej.2018.04.057.

A. Paz, D. Outeiriño, N. Pérez Guerra, and J. M. Domínguez, “Enzymatic hydrolysis of brewer’s spent grain to obtain fermentable sugars,” Bioresour Technol, vol. 275, pp. 402–409, Mar. 2019, doi: 10.1016/j.biortech.2018.12.082.

E. Mardawati, R. Andoyo, K. A. Syukra, M. Kresnowati, and Y. Bindar, “Production of xylitol from corn cob hydrolysate through acid and enzymatic hydrolysis by yeast,” in IOP Conference Series: Earth and Environmental Science, Institute of Physics Publishing, Apr. 2018. doi: 10.1088/1755-1315/141/1/012019.

E. Mardawati, A. V. Putri, T. Yuliana, S. Rahimah, S. Nurjanah, and I. Hanidah, “Effects of substrate concentration on bioethanol production from oil palm empty fruit bunches with simultaneous saccharification and fermentation (SSF),” in IOP Conference Series: Earth and Environmental Science, Institute of Physics Publishing, Feb. 2019. doi: 10.1088/1755-1315/230/1/012079.

P. Jnawali, V. Kumar, B. Tanwar, H. Hirdyani, and P. Gupta, “Enzymatic Production of Xylooligosaccharides from Brown Coconut Husk Treated with Sodium Hydroxide,” Waste Biomass Valorization, vol. 9, no. 10, pp. 1757–1766, Oct. 2018, doi: 10.1007/s12649-017-9963-4.

E. Mardawati, N. Annazhifah, N. Sukri, E. Suryadi, and B. Mandra Harahap, “Physicochemical Properties of Xylitol Crystals from Oil Palm Empty Fruit Bunches Hydrolysate,” vol. 10, no. 4, 2020.

M. Delgado, M. Navarro, A. Lázaro, S. A. E. Boyer, and E. Peuvrel-Disdier, “Triggering and acceleration of xylitol crystallization by seeding and shearing: Rheo-optical and rheological investigation,” Solar Energy Materials and Solar Cells, vol. 220, Jan. 2021, doi: 10.1016/j.solmat.2020.110840.

M. T. Asghar et al., “Processing of coconut sap into sugar syrup using rotary evaporation, microwave, and open-heat evaporation techniques,” J Sci Food Agric, vol. 100, no. 10, pp. 4012–4019, Aug. 2020, doi: 10.1002/jsfa.10446.

SON. Yudiastuti, E. Mardawati, MTAP. Kresnowati, and Y. Bindar, “Comparative study of glucose and xylose production in enzymatic hydrolysis by batch and fed-batch method,” Jurnal Teknologi pertanian, vol. 12, no. 1, pp. 79–86, 2018.

S. O. N. Yudiastuti, E. Mardawati, M. Kresnowati, and Y. Bindar, “Comparative Study of Glucose and Xylose Production in Enzymatic Hydrolysis Result By Batch and Fed-Batch Method,” Jurnal Teknotan, vol. 12, no. 1, 2018, doi: 10.24198/jt.vol12n1.9.

V. A. Vieira Queiroz et al., “A low calorie and nutritive sorghum powdered drink mix: Influence of tannin on the sensorial and functional properties,” J Cereal Sci, vol. 79, pp. 43–49, 2017, doi: 10.1016/j.jcs.2017.10.001.

S. O. N. Yudiastuti, R. Wijaya, and T. Budiati, “The effect of ozonation time and contact time of edamame washing on color changes using the continuous type ozone washing method,” IOP Conference Series : Earth and Environmental Science, 2021, doi: 10.1088/1755-1315/672/1/012066.

S. Fatma et al., “Lignocellulosic Biomass: A Sustainable Bioenergy Source for Future,” Protein Pept Lett, vol. 25, no. January 2018, doi: 10.2174/0929866525666180122144504.

E. Mardawati, A. Trirakhmadi, and M. Kresnowati, “Kinetic study on Fermentation of xylose for The Xylitol Production,” vol. 1, no. 1, pp. 1–6, 2017.

E. Mardawati, A. Werner, T. Bley, M. Kresnowati, and T. Setiadi, “The Enzymatic Hydrolysis of Oil Palm Empty Fruit Bunches to Xylose,” Journal of the Japan Institute of Energy, vol. 93, pp. 973–978, 2014.

D. Indra Wardhana, E. Ruriani, and A. Nafi, “Karakteristik Kulit Kopi Robusta Hasil Samping Pengolahan Metode Kering dari Perkebunan Kopi Rakyat di Jwa Timur,” Agritrop, vol. 17, no. 2, pp. 220–229, 2019, [Online]. Available: http://jurnal.unmuhjember.ac.id/index.php/AGRITROPEISSN

L. Pudjiastuti, T. Iswanto, A. Altway, E. O. Ningrum, and T. Widjaja, “Lignocellulosic Properties of Coffee Pulp Waste after Alkaline Hydrogen Peroxide Treatment,” in IOP Conference Series: Materials Science and Engineering, Institute of Physics Publishing, 2019. doi: 10.1088/1757-899X/543/1/012081.

D. S. Scully, A. K. Jaiswal, and N. Abu-Ghannam, “An investigation into spent coffee waste as a renewable source of bioactive compounds and industrially important sugars,” Bioengineering, vol. 3, no. 4, Dec. 2016, doi: 10.3390/bioengineering3040033.

E. Mardawati, N. Annazhifah, N. Sukri, Triyuliana, E. Suryadi, and B. M. Harahap, “Physicochemical properties of xylitol crystals from oil palm empty fruit bunches hydrolysate,” Int J Adv Sci Eng Inf Technol, vol. 10, no. 4, pp. 1646–1653, 2020, doi: 10.18517/ijaseit.10.4.11596.

Z. M. Astuti, D. Ishartani, and D. R. A. Muhammad, “Penggunaan Pemanis Rendah Kalori Stevia pada Velva Tomat (Lycopersicum esculentum mill),” Jurnal Teknologi Hasil Pertanian, vol. 14, no. 1, pp. 31–43, Feb. 2021.

F. L. Salsabela and R. Abryanto, “Analysis of Food Handler’s Knowledge of Hygiene and Sanitation Impact on Food Quality,” Proceedings of the 6th International Conference of Food, Agriculture, and Natural Resource (IC-FANRES 2021), vol. 16, pp. 310–314, 2022, doi: 10.2991/absr.k.220101.042.

https://www.alibaba.com, “AlibabaPricelist ,” https://www.alibaba.com/trade/search?spm=a2700.galleryofferlist.0.0.7c7a47060qNPTV&tab=all&searchText=xylose+sugar+xylose&viewtype=L.

https://www.sigmaaldrich.com, “Sigma Aldrich Pricelist,” https://www.sigmaaldrich.com/ID/en/search/xylose?focus=products&page=1&perpage=30&sort=relevance&term=xylose&type=product.

https://www.tokopedia.com, “Tokopedia Pricelist,” https://www.tokopedia.com/search?q=asap+cair&source=universe&st=product&srp_component_id=




How to Cite

Yudiastuti, S. O. N., Handayani, W., Sari, E. K. N., Wijaya, R., Brilliantina, A., & Slamet, A. H. H. (2024). Optimization of Crystalline Xylose Production from Coffee Cherry Processing Waste. Revista De Gestão Social E Ambiental, 18(9), e06460. https://doi.org/10.24857/rgsa.v18n9-029